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KINETIC MODELING OF MONOMERIC SUGARS FORMATION DURING THE ENZYMATIC HYDROLYSIS OF THE RESIDUE GENERATED IN THE CARRAGEENAN PRODUCTION FROM ALGAL BIOMASS

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A simple mathematical model, based on a saturation kinetics, is developed and used to interpret experimental data from the enzymatic hydrolysis of the polysaccharide fraction contained in the residue generated by the carrageenan processing of algae biomass (Kappaphycus alvarezii) for monomeric sugars production. This residue was subjected to hydrolysis with different commercial enzymatic extracts containing enzyme loads of 10 and 100 FPU/g-substrate. A detailed sequence of model development, parameter estimation, and model validation is presented. The temporal profiles of monomeric sugars concentration were derived by direct analytical integration of the mathematical model, obtaining an explicit equation over time. The model adjustment goodness was evaluated by different statistical criteria, including the Fisher’s F-test, R^2-value, and p-value and the accuracy of the model was judged acceptable. The good performance presented by the mathematical model recommends its use in further studies aimed at improving the enzymatic hydrolysis process.